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Antibodies bound to the blocking peptide now not bind for the epitope within the target protein. By comparing the staining within the blocked antibody vs . the antibody alone, you can see which staining is precise.
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This information outlines even further doable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
This guide outlines further achievable optimization of cellular and immunoassay parameters to be sure the very best results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind precisely to the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
Concentrate on Info Cyclin E belongs to your really conserved cyclin family, whose associates are characterized by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation styles which add on the temporal coordination of every mitotic celebration. Cyclin E varieties a posh with and features like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and it is degraded as cells development by way of S phase.
Overexpression of the gene has become observed in lots of tumors, which results in chromosome instability, and therefore might contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital position in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are explained. Two further splice variants ended up reported but thorough nucleotide sequence information and facts isn't however readily available.
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